sfGFP expression cassette under the control of J23110 promoter
Usage and Biology
The pictures of E. coli cells harbouring this part show a very strong sfGFP expression. In contrast, no sfGFP is observed in the absence of the promoter and the RBS (BBa_K2675080).
Figure 1. Pictures of E. coli cells harbouring an empty pSB1C3 backbone, BBa_K2675080 or this part BBa_K2675066.
We used this part as a positive control of sfGFP expression and as a negative control of promoter regulation.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 90
- 1000COMPATIBLE WITH RFC