This part is sfGFP (BBa_K2675005) fused to LVA degradation tag.
Usage and Biology
superfolder GFP (sfGFP) is a derivative of the Green Fluorescent Protein from Aequorea victoria with improved features in terms of intrinsic brightness, tolerance of circular permutation, resistance to chemical denaturants and folding kinetics at 37°C . The protein contains 13 mutations compared to wild-type (Uniprot P42212) : S2R, S30R, Y39N, F64L, S65T, S72A, F99S, N105T, Y145F, M153T, V163A, I171V & A206V.
LVA degradation tag (AANDENYALVA) is a ssrA tag that accelerates protein degradation in Escherichia coli at 37°C . It is used in synthetic biology to rapidly degrade reporter genes and thus finely observe the synthetic gene networks dynamics.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 25
- 1000COMPATIBLE WITH RFC