Composite

Part:BBa_K2673004

Designed by: Madelyn Yeh   Group: iGEM18_Tacoma_RAINmakers   (2018-10-09)


pSB1C3/PArsR-amilCP

pSB1C3/PArsR-amilCP is used to test part of the complete 2-part arsenic circuit. The part contains an E. coli-derived Arsenic Regulator promoter (PArsR), Ribosome Binding Site, spisPink chromoprotein, and Double Terminator. The expression of the amilCP chromoprotein is regulated only if ArsR is present. In that case, ArsR will repress the promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None