Composite

Part:BBa_K2673001

Designed by: Madelyn Yeh   Group: iGEM18_Tacoma_RAINmakers   (2018-10-09)


pSB1C3/PcArsR_PArsR-amilCP

pSB1C3/PcArsR_PArsR-amilCP is Tacoma RAINMaker’s arsenic detection circuit. It is a 2-part circuit that combines 2 series of promoter-gene sequences on one plasmid. The first part of the circuit, PcArsR, contains an E. coli-derived constitutive promoter (Pc), Ribosome Binding Site, Arsenic Regulator (ArsR), and Double Terminator. This sequence allows for unregulated expression of ArsR. The second part of the circuit contains an E. coli-derived Arsenic Regulator promoter (PArsR), Ribosome Binding Site, amilCP chromoprotein, and Double Terminator. The PArsR sequence has a binding site for ArsR, which is a repressor protein that blocks expression of the reporter. When Arsenic ions are present, the Arsenic Regulator proteins bind to those ions instead, allowing for amilCP chromoproteins to be produced and fluoresce blue.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None