Composite

Part:BBa_K2673000

Designed by: Madelyn Yeh   Group: iGEM18_Tacoma_RAINmakers   (2018-10-09)


pSB1C3/PcArsR_PArsR-spisPink

pSB1C3/PcArsR_PArsR-spisPink is Tacoma RAINMaker’s arsenic detection circuit. It is a 2-part circuit that combines 2 series of promoter-gene sequences on one plasmid. The first part of the circuit, PcArsR, contains an E. coli-derived constitutive promoter (Pc), Ribosome Binding Site, Arsenic Regulator (ArsR), and Double Terminator. This sequence allows for unregulated expression of ArsR. The second part of the circuit contains an E. coli-derived Arsenic Regulator promoter (PArsR), Ribosome Binding Site, spisPink chromoprotein, and Double Terminator. The PArsR sequence has a binding site for ArsR, which is a repressor protein that blocks expression of the reporter. When Arsenic ions are present, the Arsenic Regulator proteins bind to those ions instead, allowing for spisPink chromoproteins to be produced.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None