Coding

Part:BBa_K2663007

Designed by: Elizabeth Johnson   Group: iGEM18_WashU_StLouis   (2018-10-17)


Sr35 Resistance Protein Operon

This part produces the Sr35 resistance protein. It is a part of wheat's intrinsic immune response pathway to wheat stem rust fungus (Put). If it interacts with the corresponding effector from Pgt, it will trigger a hypersensitive response in wheat and prevent spreading of the infection. This part and its protein product can be used to detect the presence of certain races of Pgt. It contains a Gal1 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1703
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3330
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None