Coding

Part:BBa_K2663004

Designed by: Elizabeth Johnson   Group: iGEM18_WashU_StLouis   (2018-10-17)


AvrSr35 Avirulence Factor Operon

This part produces the AvrSr35 avirulence factor which is naturally secreted by select races of wheat stem rust fungus when infecting wheat. Since this part has been made to be produced in wheat, it will not be capable of infection in any crops. It can be used to stimulate the presence of certain races of wheat rust fungi. It contains nYFP, which produces a fluorescent signal when it interacts with cYFP. It contains a Gal1 and Tef1 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2467
    Illegal BamHI site found at 2594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
    Illegal AgeI site found at 2636
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 566
    Illegal SapI site found at 1767


[edit]
Categories
Parameters
None