Coding

Part:BBa_K2663003

Designed by: Elizabeth Johnson   Group: iGEM18_WashU_StLouis   (2018-10-17)


AvrSr35 Avirulence Factor from Wheat Stem Rust Fungus

This part produces the AvrSr35 avirulence factor which is naturally secreted by select races of wheat stem rust fungus when infecting wheat. Since this part has been made to be produced in wheat, it will not be capable of infection in any crops. It can be used to stimulate the presence of certain races of wheat rust fungi. It contains nYFP, which produces a fluorescent signal when it interacts with cYFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1910
    Illegal BamHI site found at 2037
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2079
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9
    Illegal SapI site found at 1210


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Categories
Parameters
None