Coding

Part:BBa_K2663002

Designed by: Elizabeth Johnson   Group: iGEM18_WashU_StLouis   (2018-10-17)


Sr35 Resistance Protein (CC-NBS-LRR resistance protein)

This part produces the Sr35 resistance protein. It is a part of wheat's intrinsic immune response pathway to wheat stem rust fungus (Put). If it interacts with the corresponding effector from Pgt, it will trigger a hypersensitive response in wheat and prevent spreading of the infection. This part and its protein product can be used to detect the presence of certain races of Pgt.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1146
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2773
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None