Part:BBa_K2662002
7-Epi-Sesquithujene Synthase
This gene encodes for 7-epi-sesquithujene synthase, which is derived from Terpene Synthase 4 from Zea mays cultivar B73. It catalyzes farnesyl pyrophosphate to 7-epi-sesquithujene and other sesquiterpenoids, including beta-bisabolene.
Results
Codon-optimized 7-epi-sesquithujene synthase (TPS4CO) was paired with genes encoding the mevalonate-dependent pathway, which produces farnesyl pyrophosphate, in a two plasmid system. The cultures were overlaid with n-octane to partition the sesqueterpenoid products. Octane samples were analyzed through gas chromatography-mass spectrometry to identify and quantify sesqueterpenoid products. The adjacent results show how 7-epi-sesquithujene production varied with growing conditions, such as differing optical density at the introduction of IPTG and n-octane. The results show that the amount of sesquiterpene production can vary widely with growing conditions.
Six biological replicates were tested to determine if product production was consistent under identical growing conditions. For these replicates, 7-epi-sesquithujene and beta-bisabolene production were quantified. The adjacent results show that sesquiterpenoid production was greatly varied, with observed concentration of the major product ranging between 0.52 to 92.78 mg/L. There must be a variable controlling production that has not been identified yet. In these replicates, it was observed that although production varied, 7-epi-sesquithujene is the major product and beta-bisabolene is the second major product of 7-epi-sesquithujene synthase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 58
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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