Composite

Part:BBa_K2656124

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-10-08)


pT7-GFPmut3b transcriptional unit 2

Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).

TU.
Figure 1. Transcriptional unit assembled. Golden Gote scars: standardized overhangs for promoter, RBS, CDS and terminator

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

  • BBa_K2656000: the BBa_I719005 constitutive strong promoter in its Golden Braid compatible version. Promoter T7 leads into a high protein expression.
  • BBa_K2656011: the B0034 ribosome biding site in its Golden Braid compatible version
  • BBa_K2656022: the BBa_K2656022 GFPmut3b sequence in its Golden Braid standardized version
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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