Composite

Part:BBa_K2656122

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-10-08)


pLux-GFPmut3b transcriptional unit

Transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).

TU.
Figure 1. Transcriptional unit assembled. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator

This transcriptional unit is composed of the following standardized parts from our Part Collection:


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None