Coding

Part:BBa_K2656016

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-09-23)


LuxR TF Coding Sequence

domestication.
Figure 1. DNA basic parts domestication. Third construction corresponds with CDS Basic Part adaptation into the GoldenBraid grammar.

Part BBa_K2656016 is the transcription factor LuxR coding sequence BBa_C0062 compatible with both Biobrick and GoldenBraid 3.0 assembly methods. It acts on the lux promoters when activated by Acyl-homoserine-lactone. It can be combined with other compatible parts from our Valencia UPV IGEM 2018 Printeria Collection to assemble transcriptional units with the Golden Gate assembly protocol .

This part used to construct the composite part BBa_K2656114. Composite part BBa_K2656114 was then used together with BBa_K2656116 in order to characterize the pLux repressible promoter BBa_K2656002.

By using this experimental protocol and inducing at different AHL concentrations, we have obtained the following experimental measures (in blue dots). These measurements were compared with our constitutive AHL-LuxR model to test it.

AHL experiment.
Figure 3. AHL induction experiment and comparison with our model simulation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/transcriptionalregulator/activator
//cds/transcriptionalregulator/repressor
//function/cellsignalling
//function/regulation/transcriptional
Parameters
None