Part:BBa_K2656015

E protein from Escherichia phage phiX174 Coding Sequence

Figure 1. DNA basic parts domestication. Third construction corresponds with CDS Basic Part adaptation into the GoldenBraid grammar.

Part BBa_K2656015 is the Lysis gene from the enterobacteria phage phiX174 coding sequence. The lysis gene E of coliphage PhiX174 encodes a 91-amino acid (aa) residue protein of approximately 10 kDa that mediates inhibition of peptidoglycan biosynthesis of Gram-negative bacteria membrane (1).

It can be use as part of synchronized lysis circuits, so generate oscillations in the densities of Escherichia coli populations by simple designing synthetic suicide circuits containing quorum components and a lysis gene (2).

This basic part has been sequence adapted to be both compatible with the Biobrick and the [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods.

Thus, it can be combined with other Golden Gate standardized basic parts, such as the ones from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection], to assemble transcriptional units in a BsaI one-pot reaction following the Golden Gate assembly protocol .

References

1. Bernhardt, T. G., Roof, W. D., & Young, R. (2000). Genetic evidence that the bacteriophage φX174 lysis protein inhibits cell wall synthesis. Proceedings of the National Academy of Sciences of the United States of America, 97(8), 4297–4302.

2. Oscillations by Minimal Bacterial Suicide Circuits Reveal Hidden Facets of Host-Circuit Physiology Marguet P, Tanouchi Y, Spitz E, Smith C, You L (2010) Oscillations by Minimal Bacterial Suicide Circuits Reveal Hidden Facets of Host-Circuit Physiology. PLOS ONE 5(7): e11909.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]