Composite

Part:BBa_K2652005

Designed by: Siyun Xiong   Group: iGEM18_HBUT-China   (2018-10-08)


ncrB, pncrA, luxCDABE, nikABCDE

This part is our complete genetic line. By expressing NikABCDE, more nickel ions enter the cell, and more nickel ions will bind to NcrB, resulting in more expression of LuxCDABE and enhancing the bioluminescence value we detected. As a result, the accuracy and sensitivity of our inspection system has been improved.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 5105
    Illegal NheI site found at 5128
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 10957
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1621
    Illegal AgeI site found at 3622
    Illegal AgeI site found at 8614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 9133


[edit]
Categories
Parameters
None