Composite

Part:BBa_K2652004

Designed by: Siyun Xiong   Group: iGEM18_HBUT-China   (2018-10-08)


Nickel ion detection


This section contains ncrB, pncrA and the reporter gene luxCDABE we inserted. LuxCDABE is a bioluminescent gene whose protein emits light using chemical energy in the living body. We measured the concentration of extracellular nickel ions by detecting the bioluminescence intensity of LuxCDABE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5859
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3516
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4035


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Categories
Parameters
None