Composite

Part:BBa_K2652003

Designed by: Siyun Xiong   Group: iGEM18_HBUT-China   (2018-10-08)


channel element

This part carries the nickel ion channel protein gene nikABCDE (actually 5 genes, our figure is simplified to one for convenience). NikA is in the periplasm between the cell membrane and the cell wall. It can capture and bind to free nickel ions and transport it to NikB and NikC located on the cell membrane. NikB and NikC have binding sites for nickel ions. NikD and NikE can be combined with ATP provides energy for the transport of nickel ions. When ATP is not bound, the binding site of nickel ion is exposed to the outside of the cell membrane. After ATP binding, the protein structure of NikB and NikC changes, and the binding site of nickel ion is transferred to the intracellular measurement, thereby completing the transmembrane transport of nickel ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1621
    Illegal AgeI site found at 3622
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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