Composite

Part:BBa_K2652002

Designed by: Siyun Xiong   Group: iGEM18_HBUT-China   (2018-10-08)


restriction

This part contains the important gene ncrB, the important promoter pncrA, and the restriction site StuI. After expression of the protein NcrB, it will bind to pncrA, the function of pncrA is inhibited, and the expression of downstream genes cannot be initiated. However, when nickel ions bind to the NcrB protein, the inhibition is released and the downstream gene begins to express. Here we designed the cleavage site StuI so that we can insert the reporter gene luxCDABE, which also using the protocol of the Gipson clone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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