Composite

Part:BBa_K2649017

Designed by: Alexis Schneider   Group: iGEM18_MIT   (2018-10-12)


hEF1a:iRFP-720

The iRFP-720 fluorescent protein driven by hEF1a promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
    Illegal PstI site found at 1648
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
    Illegal PstI site found at 1648
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal BglII site found at 591
    Illegal BamHI site found at 1197
    Illegal BamHI site found at 1234
    Illegal XhoI site found at 990
    Illegal XhoI site found at 1570
    Illegal XhoI site found at 1576
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
    Illegal PstI site found at 1648
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
    Illegal PstI site found at 1648
    Illegal NgoMIV site found at 725
    Illegal NgoMIV site found at 1351
    Illegal AgeI site found at 103
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None