Coding

Part:BBa_K2647000

Designed by: Denise Pezzutto, Jessica Koski   Group: iGEM18_Aalto-Helsinki   (2018-10-08)

This part encodes for 6 repeats of dragline spider silk protein ADF-3 from garden spider Araneus diadematus. This part contains BsaI sites in the beginning and in the end of the sequence making it possible to use this sequence in the scarless assembly with Golden Gate.

For more details on the production and usage of this part please check the composite part page: BBa_K2647001, BBa_K2647003, BBa_K2647004


ADF-3 spider silk repetitive sequences


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 820
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