Coding

Part:BBa_K2641000

Designed by: Selina Beck   Group: iGEM18_FAU_Erlangen   (2018-08-24)


CDS of S-layer protein PS2

CDS for S-layer protein of Corynebacterium glutamicum ATCC 14067. For use of heterologous overexpression in Corynebacterium glutamicum strains with a natural S-layer mutation if cloned into a specific vector.

Further usage as template for fusions at the N-terminus of the S-layer.

Sequence does not conatin an affinity tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1387
    Illegal XhoI site found at 214
    Illegal XhoI site found at 832
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 202
    Illegal AgeI site found at 1369
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1366
    Illegal SapI site found at 613
    Illegal SapI site found at 825


[edit]
Categories
Parameters
None