Part:BBa_K2638759

Seamless siRNA Expression Vektor with an Hfq binding tag and OmpA 5'-UTR

This expression vektor is part of the siRNA measurement system. through the use of the Bbs I restriction enzyme, siRNAs can be ordered as oligos and integrated in the vector without leaving scars. This minimizes changes in the transkribed siRNA by scars or spacer nucleotides between sequences This vektor contains a 3' Hfq binding Tag, and a 5' Protective sequenz from the OmpA 5'-untranslated region which forms secondary structure to protect the siRNA from beeing brocken down. Using the Bbs I restriction enzyme siRNAs can be ordered as overlapping oligonuclotides with a 4 BP overlap to the vector. The Hfq Protein facilitates binding of siRNA and mRNA and raises the local concentration of siRNAs.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  Illegal XhoI site found at 1565
  Illegal XhoI site found at 1864
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1869
  Illegal AgeI site found at 979
  Illegal AgeI site found at 1771
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961