Generator

Part:BBa_K2638758

Designed by: Antonin Lenzen   Group: iGEM18_Bielefeld-CeBiTec   (2018-10-10)


Seamless siRNA Expression Vektor for Omp-Utr protected siRNAs

This expression vektor is a part of the siRNA measurement system. By using the Bbs I restriction enzyme siRNAs can be ordered as oligonucleotides and seamlessly integrated into the vector. It is important that this vektor was build without scars, as overlapping bases directly influence the transkribed riRNA. This vektor includes the 5'- untranslated region of the OmpA gene, which forms protective structures that protects the mRNA from beeing brocken down by 5' RNAses.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1771
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None