Generator

Part:BBa_K2638702

Designed by: Antonin Lenzen   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


Seamless siRNA Expression Vektor for Hfq binding siRNAs

This part can generate siRNAs which can be seamlessly cloned into the Vektor by means of Golden Gate Assembly using the restriction enzyme BbsI. The use of a Golden Gate Casette containing the LacZalpha complementation system can be used for fast screening for positive clones after Transformation. This Part uses the Inducible pBad/araC promoter BBa_I0500 for the transkription of the siRNA. The transcription is terminated by the Terminator BBa_J61048. It contains the Hfq binding Tag from Biobrick BBa_K2638700.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1448
    Illegal XhoI site found at 1747
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1752
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None