Coding
Part:BBa_K2638101
Designed by: Johannes Ruhnau Group: iGEM18_Bielefeld-CeBiTec (2018-09-02)
alkyl hydroperoxide reductase, F52a subunit (ahpF)
AhpF belongs to the family of pyridine nucleotide-disulphide oxidoreductases and channels electrons from NAD(P)H towards the AhpC (https://parts.igem.org/Part:BBa_K2638100, BBa_K2638100) component which is able to reduce hydrogen peroxide and organic hydroperoxides. AhpF forms together with AhpC the holoenzyme Ahp / AhpR. The protein can bind NADH or NADPH in order to gain electrons for the reduction of oxidized AhpC. The active site is a redox-active disulfide bond. FAD is needed as a cofactor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 289
Illegal AgeI site found at 712
Illegal AgeI site found at 964 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 613
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Categories
Parameters
None |