Coding

Part:BBa_K2638000

Designed by: Erika Schneider   Group: iGEM18_Bielefeld-CeBiTec   (2018-08-26)


HmtA

HmtA (heavy metal transporter A) is a P-type ATPase, importing Zn(II) and Cu(II) ions from the periplasm into the cytoplasm using ATP. Contains the coding sequence (CDS). This part fulfills the same function as HmtA from iGEM Groningen 2009 (BBa_K190018), but is not from the Pseudomonas aeruginosa genome, but the homologue gene from Pseudomonas brassicacearum. Also it does not contain a PstI restriction site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 917
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 507
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 873
    Illegal NgoMIV site found at 1220
    Illegal NgoMIV site found at 1288
    Illegal NgoMIV site found at 1609
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 346


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Categories
Parameters
None