Part:BBa_K2635000
TRE-3G promoter-mCherry
This is one of the output signals in our reporter cell during experiments. As the transcription factor, tTA, binds to TRE3G promoter regulating the downstream gene, mCherry is expressed. In our design, a series of reaction followed by the presence of GFP will release tTA, which will be translocated into the nucleus.
GFP binds to transfected 293T cells and induces mCherry expression. 293T cells transfected with pCAG-GBP-tTA and pTRE3G-mCherry were incubated with GFP (100 ng/ml) for 24 hrs. Subsequently, cells were fixed by 4% paraformaldehyde and stained with DAPI (blue, DAPI). Arrows indicate GFP and mCherry double positive cells. The hallow arrowhead indicate GFP positive and mCherry negative cells.
We ran further experiments and confirm the complete design.(http://2018.igem.org/Team:NTHU_Formosa/Results)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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