RNA

Part:BBa_K2624006

Designed by: Kexin Cao, Junqing Zhang   Group: iGEM18_CPU_CHINA   (2018-08-28)


MAP4K4 miRNA sponge

MicroRNA sponges are transcripts that competitively inhibit the target miRNA. In this case it targets the miRNA(MAP4K4). If you are trying to express this miRNA in your cells, you probably need it for its regulation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 178
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 178
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

The 2018 CPU_CHINA project is a gene therapy strategy based on conditional RNA interference. For the RNAi to be also controllable by a small MV drug, they added a tet-off system, and this miRNA sponge is the effective product that down-regulates the miRNA that could damage cell biology. Apart from ‘absorbing’ miRNA, we don’t see how it could be otherwise used for.

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