Reporter
NG5

Part:BBa_K2623025

Designed by: Yunyun Hu   Group: iGEM18_XMU-China   (2018-10-02)


Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1.

Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we insert TorA leader sequence to the circuitry and conjugate mRFP1 with L7Ae to validate the SpyTag/SpyCatcher bioconjugation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1168
    Illegal NheI site found at 1191
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2325
    Illegal AgeI site found at 2437
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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