Coding
CikA

Part:BBa_K2623005

Designed by: Yuhan Qin   Group: iGEM18_XMU-China   (2018-09-28)


CikA coding region

Summary

It is a component of the Kai circuit, which dephosphorylates the phosphorylated RpaA, preventing phosphorylated RpaA from continually activating transcription of related promoters. A separate fragment is 2265bp.

Structure of CikA

CikA.png

CikA's function in phosphorylation pathway


Fig.1 Simplified model of the central circadian oscillator and output activities.[1]

Reference

[1]Joseph S. Markson,Joseph R. Piechura,Anna M. Puszynska,Erin K. O’Shea. Circadian Control of Global Gene Expression by the Cyanobacterial Master Regulator RpaA[J]. Cell,2013,155(6).
More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 127
    Illegal BamHI site found at 2187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None