Composite

Part:BBa_K2622039

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)


BamA-His composite

Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Constitutes, with BamD, the core component of the assembly machinery. Tagged with His-6x, under control of T7 promoter and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2521
    Illegal NotI site found at 2460
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 366
    Illegal XhoI site found at 2172
    Illegal XhoI site found at 2469
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1293
    Illegal AgeI site found at 1812
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None