Part:BBa_K2619105
nano luciferase with 3 MS2 stem loop sequence and 4*VEEV stabilizing sequence on 3' UTR region
MS2 stem loop can be added to 3'-UTR region of actively loading RNA to bind to RNA binding domain of packaging device 2 (MS2), to increase the assembly efficiency of MS2 stem loop with MS2 packaging device, we used three times stem loop. VEEV would stable mRNA would stable mRNA during transporting process and transcribing in target cells. By combining these two sequences on 3' UTR region of a target gene, this gene can be packaged into exosome and delivered to target cell and exist for more than 36 hours. The reporter gene in this part is Nano luciferase, which is a very small luciferase that is only 19kDa. by adding luciferase substrate, Nano luciferase will give off light that can be detected by enzyme-labeled instruments. Therefore this part is designed for researchers to exam whether the packaging device could work.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 253
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 69
- 1000COMPATIBLE WITH RFC[1000]
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