Part:BBa_K2619104

Nanoluciferase with C/D box sequence and 4*VEEV RNA stabilizing sequence at the 3' UTR

C/D box-VEEV can be added to 3'-UTR region of actively loading RNA to bind to RNA binding domain of packaging device 1 (L7AE). VEEV would stable mRNA during transporting process and transcribing in target cells. By combining these two sequences on 3' UTR region of a target gene, this gene can be packaged into exosome and delivered to target cell and exist for more than 36 hours. The reporter gene in this part is Nano luciferase, which is a very small luciferase that is only 19kDa. by adding luciferase substrate, Nano luciferase will give off light that can be detected by enzyme-labeled instruments. Therefore this part is designed for researchers to exam whether the packaging device could work.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 253
  Illegal XhoI site found at 611
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 69
  
• 1000
  COMPATIBLE WITH RFC[1000]