RNA

Part:BBa_K2619005

Designed by: Peng Chai   Group: iGEM18_XJTLU-CHINA   (2018-09-07)


C/D box

C/D box in our project is an archaeal member of the family of C/D box which can act as the guide RNA of Ribonucleoprotein. The sequence of this part comes from Haloferax volcanll. This C/D box contained the structure of k-loop RNA motif which can be recognized by L7Ae. By inserting it to the 3'-UTR of cargo mRNA, mRNA can be loaded by CD63-L7Ae (BBa_K2619101) into engineered exsomes in the exosome biogenesis process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 67
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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