Part:BBa_K2610037
pCspA-GFP
This composite part features the regulatory part promoter CspA (BBa_K2610036) and the fluorescent protein GFP (BBa_E0040). It can be used to visualize upregulation of CspA. CspA, or cold shock protein A, binds to the CAAT-containing regions of cold-inducible promoters of the H-NS and GyrA proteins.
Usage and Biology
We, iGEM Leiden 2018, have designed this composite part as part of our project Fifty Shades of Stress. This reporter part allowed us to detect stress-induced changes in SoxS transcription. We have created this part in order to amplify the fluorescent signal measured after pCspA-GFP activation or downregulation (Figure 1). It is evident that CspA expression is repressed with various antimicrobial agents such as streptomycin, kanamycin and tetracycline.
Figure 1. Median Fluorescence Intensity (MFI) in AU after 4 hour incubation with antimicrobial agents in various concentrations.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 945
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