Regulatory

Part:BBa_K2610028

Designed by: Charlotte de Ceuninck van Capelle, Laurens ter Haar   Group: iGEM18_Leiden   (2018-10-10)


pSoxS-T7 RNA polymerase

This composite part features the basic part promoter SoxS (BBa_K2610030) and the T7 RNA polymerase (BBa_K1706007). T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA in the 5'→ 3' direction. This part allows for transcription of the RNA polymerase gene upon activation of the SoxS/SoxR pathway in response to bacterial cell stress in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1996


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Categories
Parameters
None