Part:BBa_K2610025

pSpy

Spy, an ATP-independent chaperone, is highly upregulated in the presence of protein unfolding agent ethanol. It decreases protein degradation.

Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5α cells for several hours.

Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the Spy promoter is activated by nalidixic acid, ethanol and chloramphenicol. This is indicative of a similar mechanism of actions for these compounds.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]