Part:BBa_K2610022
pCpxR
The CpxR protein is a member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes.(Batchelor et al, 2005)
For more information please refer to our [http://2018.igem.org/Team:Leiden/Results Results page]
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the CpxR promoter is only moderately activated by hydrogen peroxide.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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