Part:BBa_K2610010

pRecN

The RecA protein is required for the repair of double strand breaks in bacterial DNA. It has been shown that RecN is upregulated in E.coli after administration of known carcinogenic agent MNNG. (Norman et al, 2004) (Finch et al, 1985)

Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.

Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the RecN promoter is not affected by the tested antibiotics.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]