Regulatory

Part:BBa_K2610009

Designed by: Charlotte de Ceuninck van Capelle, Marjolein Crooijmans   Group: iGEM18_Leiden   (2018-10-09)


pRecA

Protein RecA allows for homologous recombination and is essential in the repair and maintenance of E.coli DNA. It has been shown that RecA is upregulated in E.coli after administration of known carcinogenic agent MNNG. (Norman et al, 2004)


Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.

T--Leiden--heatmap.png

Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the RecA promoter is severely downregulated by multiple antibiotics.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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