Regulatory
Part:BBa_K2610006
Designed by: Charlotte de Ceuninck van Capelle, Marjolein Crooijmans Group: iGEM18_Leiden (2018-10-09)
pMalK
The gene MalK encodes for the maltose/maltodextrin import ATP-binding protein MalK, allowing for active transport of maltose across the cell membrane of E.coli. (Maurer et al, 2004)
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1)
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the MalK promoter is downregulated by a variety of antibiotics.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
| None |