Composite

Part:BBa_K259014

Designed by: Petros Mina   Group: iGEM09_BCCS-Bristol   (2009-10-15)


AraC-RBS-FhuA-GFP

The part was designed to assess if FhuA can be delivered to the Outer Membrane and to Outer Membrane vesicles in E.coli.

For delivery purposes please use part FhuA BBa_K259000 or AraC-RBS-FhuA BBa_K259010 and create your own protein fusions using Bioscaffolds like BpuEI-BseRI BBa_K259002

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
    Illegal AgeI site found at 1048
    Illegal AgeI site found at 1186
    Illegal AgeI site found at 1591
    Illegal AgeI site found at 2365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3263
    Illegal SapI site found at 107
    Illegal SapI.rc site found at 1090


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Parameters
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