Composite

Part:BBa_K259013

Designed by: Petros Mina   Group: iGEM09_BCCS-Bristol   (2009-10-15)


AraC-RBS-GFP-Terminator

A part used to quantify the AraC-RBS construct BBa_K259007 created by the BCCS-Bristol iGEM'09 team.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1010
    Illegal SapI site found at 107


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//rbs/prokaryote/constitutive/anderson
//rbs/prokaryotic/constitutive/miscellaneous
//regulation/multiple
//regulation/negative
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
regulator
Parameters
biologyT7TE-LuxIA
controlaraC
directionBidirectional
emission
emit511
excitation
excite501
forward_efficiency0.604[CC]/0.96[JK]
lum
negative_regulators
o_h
o_l
positive_regulators
proteinGFPmut3b
rbs
reversed_version2145
reverse_efficiency0.86[JK]
tagNone