Composite

Part:BBa_K259005

Designed by: Petros Mina   Group: iGEM09_BCCS-Bristol   (2009-09-03)


AraC Rheostat Promoter

The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins.

When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the expression of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth.

This composite part does not have an LVA degradation tag at its end, hence can be used with all prokaryotic assemblies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 107


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/multiple
//regulation/negative
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
regulator
transcriptional
Parameters
biology
controlaraC
directionForward
negative_regulators
o_h
o_l
positive_regulators