Part:BBa_K259001

fiu � Outer Membrane Ferric Iron Transporter

Biology of Fiu

The fiu gene encodes for an outer membrane transporter found in E.coli, a gram negative bacterium. It is involved in the active transport (energy dependent uptake) of ferric iron from the extracellular environment. This outer membrane receptor can only bind ferric iron and is a catecholate siderophore. For uptake into the cell to be completed fiu has to complex with another protein known as TonB (a transperiplasmic protein) that will carry out the active transport by using ATP as an energy source.

What this Biobrick gives you upon expression

This biobrick encodes ONLY for fiu.

Fiu Statistics

Fiu belongs to the family of Ton-B dependent receptors and is related to fhuA, hence it also has a beta-barrel structure.

Chassis	Monomer/Multimer	Aminoacid Length/Weight	Localisation	Structure
E.Coli	Monomer	760/81.96kDa	Outer Membrane/OMV	beta-barrel

References

[http://ecoliwiki.net/colipedia/index.php/fiu:Gene_Product%28s%29#Domains.2FMotifs.2FModification_Sites E.Coli wiki net]

Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)

[http://www.ecogene.org/geneInfo.php?eg_id=EG13317 Ecogene number EG13317]

[http://www.uniprot.org/uniprot/P75780 UniProt Accession number P75780]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 682
  Illegal AgeI site found at 820
  Illegal AgeI site found at 1225
  Illegal AgeI site found at 1999
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 724