Part:BBa_K259000

fhuA - Outer membrane transporter for ferrichrome-iron

The liganded/unliganded forms of fhuA. The image is adapted from Locher K.P. et al.(1998) (see references below).

Biology of fhuA

fhuA is an outer membrane (OM) protein that is found in the gram negative bacterium, E.coli. It is involved in the active-transport (energy-dependent uptake) of ferrichrome-iron from the extracellular environment. However fhuA is only able to bind ferrichrome-iron (i.e. ferrichrome iron is a ligand of fhuA protein). For the uptake to be completed fhuA needs to form a complex with another protein, tonB that carries out the energy-dependent uptake by using ATP as substrate to undergo the relevant conformational changes.

A plug blocks the pore to prevent membrane leakage. This plug is found in the extracellular environment.

What this Biobrick gives you upon expression

This biobrick encodes ONLY for fhuA.

fhuA Statistics

fhuA is a beta-barell protein (22 anti-parallel beta-strands) and is composed of 747 aminoacids. This receptor protein may be used as an attachment point by some colicins.

Chassis	Monomer/Multimer	Aminoacid Length/Weight	Localisation	Structure
E.Coli	Monomer	747/82.182kDa	Outer Membrane	beta-barrel

References

Locher K.P., Rees B., Koebnik R., Mitschler A., Moulinier L., Rosenusch J.P. and Moras D. (1998),Transmembrane signalling across the Ligand-Gated FhuAReceptor: Crystal structures of Free and Ferrichrome-Bound States reveal allosteric changes. Cell 95,771-778.

[http://www.uniprot.org/uniprot/P06971 Uniprot access number P06971]

[http://ecogene.org/geneInfo.php?eg_id=EG10302 EcoGene Access Number EG10302]

[http://ecoliwiki.net/colipedia/index.php/fhuA:On_One_Page E.coli wikinet]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 682
  Illegal AgeI site found at 820
  Illegal AgeI site found at 1225
  Illegal AgeI site found at 1999
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 724