Composite
Hly-OmpT

Part:BBa_K2588030

Designed by: Daniel Wedemeyer   Group: iGEM18_Hamburg   (2018-10-05)


Hly secretion system and OmpT generator, arabinose-induced

We improved the secretion system first introduced by UNICAMP EMSE Brazil team, BBa_K554013, for our use as a secretion device for our toxin BjaIT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1435
    Illegal BglII site found at 2098
    Illegal BamHI site found at 65
    Illegal BamHI site found at 4142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 365
    Illegal AgeI site found at 3602
    Illegal AgeI site found at 3659
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None