Coding
tesA

Part:BBa_K2588014

Designed by: Bjarne Klopprogge   Group: iGEM18_Hamburg   (2018-10-05)


Fatty Acyl-ACP thioesterase 1

Usage and Biology

The thioesterase I catalyzes the reaction of fatty acyl-ACP to the according fatty acid in E. coli.1 The most active substrates of the thioesterase are fatty acyl-CoA thioesters with a carbon chain length of 14 to 18.2

The thioesterase I was used in our myristic acid synthesis module, BBa_K2588022.

References

  1. Xu, P. et al. Modular optimization of multi-gene pathways for fatty acids production in E. coli. Nat. Commun. (2013). doi:10.1038/ncomms2425
  2. Bonner, W. M. & Bloch, K. Purification and properties of fatty acyl thioesterase I from Escherichia coli. J. Biol. Chem. 247, 3123–33 (1972).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None