Part:BBa_K2588010

Acetyl-CoA carboxyltransferase subunit alpha

Usage and Biology

The acetyl-CoA carboxylase subunit alpha is part of the acetyl-CoA carboxylase ACC, which is the major regulator for fatty acid biosynthesis in E. coli. In this complex it forms an α₂β₂ heterotetramer with the acetyl-CoA carboxylase subunit beta encoded by the accA gene.¹ This tetramer catalyzes the reaction of acetyl-CoA to malonyl-CoA by transferring a carboxyl group from carboxybiotin.²

The acetyl-CoA carboxyltransferase was used in our myristic acid synthesis module, BBa_K2588022.

References

1. Li, S. J. & Cronan, J. E. The genes encoding the two carboxyltransferase subunits of Escherichia coli acetyl-CoA carboxylase. J. Biol. Chem. 267, 16841–7 (1992).
2. Polakis, S. E., Guchhait, R. B., Zwergel, E. E., Lane, M. D. & Cooper, T. G. Acetyl coenzyme A carboxylase system of Escherichia coli. Studies on the mechanisms of the biotin carboxylase- and carboxyltransferase-catalyzed reactions. J. Biol. Chem. 249, 6657–67 (1974).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 579
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 517
  Illegal SapI.rc site found at 714