Regulatory

Part:BBa_K2586013

Designed by: Janek Meißner   Group: iGEM18_Goettingen   (2018-09-13)


Palf4-RBS-AroA*: Palf4 linked to a RBS linked to AroA*

This part is a composite part made from (BBa_K2586000) ; (BBa_K2586008) and (BBa_K2586001).
It connects the Palf4 Promoter to a RBS and also makes a connection to the resistant AroA* variant. This composite part was used within the model bacterium Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 403
    Illegal AgeI site found at 844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1078


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
control